In this edition of multiplex tissue image of the month, meiosis-associated BEN domain containing 2 (gene: BEND2) is showcased within developing spermatocytes in testis.

The multiplex immunohistochemistry (mIHC/IF) spermatocytes panel can be used to identify proteins that are expressed at specific stages of spermatogenesis. Using this panel, BEND2 - a protein that has been linked to the regulation of the chromatin state during spermatogenesis in mice - is mainly localized (green) to the nucleus of spermatocytes in cell state 2. Other visualized cells include spermatocytes at cell state 3 (magenta), as well as early (white) and late (red) spermatids.

The spatial expression pattern is supported by single-cell RNA sequencing data for BEND2 at the Human Protein Atlas that shows elevated expression in early primary spermatocytes.

For more details about the mIHC/IF technique visit our method page.